Effect of interferon receptor 1 silenced human diploid MRC⁃5 cell line on replication of varicella⁃zoster virus / 中国生物制品学杂志

@#Abstract：Objective To improve the replication level of varicella⁃zoster virus（VZV）in human diploid cell line MRC⁃5 and increase the yield of VZV vaccine by reducing the expression of interferon（IFN）related genes via optimizing the cell line MRC⁃5. Methods Interferon receptor 1（IFNAR1）silenced MRC⁃5 cell line（MRC⁃5IFNAR1⁃）was constructed by CRISPR／Cas9 gene editing technology，which was determined for the relative expression of IFNAR1 mRNA，and for those of mRNA of IFN related genes IFNβ and OAS1 after VZV infection by qRT⁃PCR to evaluate the effect of gene silencing. Gene mutation sequences were further identified by sequencing of the silenced sites. The replication of VZV in MRC⁃5 and MRC⁃5IFNAR1⁃ cell lines was compared 168 h after VZV infection by using qRT⁃PCR and plaque formation unit（PFU）assay， to evaluate the effect of MRC⁃5IFNAR1⁃cell line on VZV replication. Results The growth status of MRC⁃5IFNAR1⁃ cell line wasconsistent with that of MRC ⁃ 5 cells，and the relative expression of IFNAR1 mRNA decreased by 73%；The relative expressions of IFNβ and OAS1 mRNA in MRC⁃5IFNAR1⁃ cell line were 61% and 90% lower than those in MRC⁃ 5 cells respectively after VZV infection；In addition，168 h after VZV infection，the level of DNA replication and the titer of VZV increased by 5. 7 folds and 4 folds respectively. Conclusion The successful establishment of MRC⁃5IFNAR1⁃ cell line may be a potential scheme to increase the yield of vaccines based on human diploid cells，and provided a reference for expanding production of VZV vaccine.

A comparative study of the clinical characteristics of spotted fever and severe fever with thrombocytopenia syndrome / 中国热带医学

@#Abstract: Objective To investigate the differences in epidemiological and clinical characteristics of patients with spotted fever (SF) and severe fever with thrombocytopenia syndrome (SFTS). Methods A total of 86 patients with SF and 113 patients with SFTS who were laboratory-confirmed in the second-level and above hospitals in Lu'an City from January 2017 to January 2022 were selected. The basic data, epidemiological history, clinical data and laboratory test results of the two diseases were retrospectively analyzed for comparison. Results The proportion of male in SF group was 32.56% (28/86), and the proportion of male in SFTS group was 53.98% (61/113), the difference was statistically significant (χ2=9.067, P<0.01). The proportions of abdominal pain and diarrhea in the SF group were (3.49%, 3/83) and (21.24%, 24/113), which were significantly lower than corresponding (6.98%, 6/86) and (46.90%, 53/113) in the SFTS group (χ2=13.121, 37.322, P<0.01). The incidences of rash and eschar in SF group were 95.35% (82/86) and 20.93% (18/86), which were significantly higher than corresponding 1.77% (2/113) and 0.88% (1/113) in SFTS group (χ2=175.311, 22.721, P<0.01). The levels of leukocytes, platelets and C-reactive protein in the SF group were significantly higher than those in the SFTS group, and the levels of transaminase, lactate dehydrogenase and D-dimer were significantly lower than those in the SFTS group, and the differences were statistically significant (all P<0.05). Conclusions The rash and inflammatory reaction are more obvious in SF patients, while the liver function, myocardial function and coagulation function are significantly impaired in SFTS patients.

The construction of sports public service system for the elderly from the perspective of healthy aging / Estructura del sistema de servicios públicos para los deportes de edad en el contexto del envejecimiento saludable / Construção de um sistema de serviço público de desportos para idosos sob a perspectiva do envelhecimento saudável

ABSTRACT With the gradual improvement of people's quality of life, the average life expectancy of our country has been extended. It is estimated that the total number of the elderly population in China will exceed 250 million by the end of 2020. Therefore, promoting healthy aging is a necessary measure to cope with the coming aging society in China, and physical exercise is an important method to keep the physical and mental health of the elderly. In order to promote the development of healthy aging in China, this study focused on the current level of public sports services for the elderly. This study uses the expert consultation method to test the rationality of the evaluation index, and uses the method of questionnaire survey to score each evaluation index, and uses the analytic hierarchy process (AHP) to calculate the weight of the index. Finally, the paper takes the public sports service of the elderly in a city as an example to verify. The results show that the level of sports public service for the elderly in this city is only 77,928. Especially the two aspects of allocation of sports venues and the use of funds are the most unsatisfactory ones. It can be targeted to improve these two aspects, so as to improve the level of public sports services for the elderly. It is hoped that, through this study, we can provide some reference for improving the level of sports public service for the elderly from the perspective of healthy aging.

RESUMO Com a melhoria gradual da qualidade de vida das pessoas, a expectativa média de vida do nosso país foi ampliada. Estima-se que o número total de pessoas idosas na China excederá 250 milhões até o final de 2020. Por isso, promover um envelhecimento saudável é uma medida necessária para lidar com a futura população idosa na China, e o exercício físico é um método importante para manter a saúde física e mental dos idosos. A fim de promover o desenvolvimento de um envelhecimento saudável na China, este estudo centrou-se no nível atual de serviços desportivos públicos para os idosos. Este trabalho utiliza o método de consulta de especialistas para testar a racionalidade do índice de avaliação, e utiliza o método de levantamento por questionário para marcar cada índice de avaliação, e utiliza o processo hierárquico analítico (AHP) para calcular o peso do índice. Finalmente, a pesquisa toma como exemplo o serviço público de desportos dos idosos numa cidade. Os resultados mostram que o nível de serviço público desportivo para os idosos nesta cidade é apenas 77.928. Em especial, os aspectos da atribuição de espaços desportivos e a utilização de recursos financeiros são os mais insatisfatórios. Pode visar-se melhorar esses dois aspectos, de modo a melhorar o nível dos serviços desportivos públicos para os idosos. Espera-se que, através deste estudo, possamos oferecer uma referência para melhorar o nível de serviço público desportivo para os idosos sob a perspectiva do envelhecimento saudável.

RESUMEN Con la mejora paulatina de la calidad de vida de las personas se ha ampliado la esperanza de vida media de nuestro país. Se estima que el número total de personas añosas en China superará los 250 millones a finales de 2020. Por lo tanto, promover un envejecimiento saludable es una medida necesaria para hacer frente al aumento de la edad de la sociedad en China. El ejercicio físico es un método importante para mantener la salud física y mental de las personas mayores. Con el fin de promover el desarrollo del envejecimiento saludable en China, este estudio se centró en el nivel actual de los servicios deportivos públicos para las personas añosas. Este estudio utiliza la consulta de expertos para probar la racionalidad del índice de evaluación, la encuesta de cuestionario para calificar cada índice de evaluación, y el proceso de jerarquía analítica (PJA) para calcular el peso del índice. Finalmente, el trabajo toma como ejemplo a ser verificado, el servicio público deportivo de la tercera edad en una ciudad determinada. Los resultados muestran que el nivel de servicio público deportivo para personas añosas en esa ciudad es de solo 77.928. Especialmente los aspectos de la asignación de instalaciones deportivas y del uso de fondos son los más insatisfactorios. Se debe apuntar a mejorar estos dos aspectos, a fin de conseguir un mejor nivel de servicios deportivos públicos para las personas añosas. Esperamos que, a través de este estudio, podamos proporcionar una referencia para mejorar el nivel de servicio público deportivo para las personas añosas desde la perspectiva del envejecimiento saludable.

Enhanced pyruvic acid yield in an osmotic stress-resistant mutant of Yarrowia lipolytica

BACKGROUND: Pyruvic acid (PA), a vital α-oxocarboxylic acid, plays an important role in energy and carbon metabolism. The oleaginous yeast Yarrowia lipolytica (Y. lipolytica) has considerable potential for the production of PA. An increased NaCl concentration reportedly increases the biomass and PA yield of Y. lipolytica. RESULTS: To increase the yield of PA, the NaCl-tolerant Y. lipolytica A4 mutant was produced using the atmospheric and room temperature plasma method of mutation. The A4 mutant showed growth on medium containing 160 g/L NaCl. The PA yield of the A4 mutant reached 97.2 g/L at 120 h (0.795 g/g glycerol) in a 20-L fermenter with glycerol as the sole carbon source, which was 28.9% higher than that of the parental strain. CONCLUSION: The PA yield from Y. lipolytica can be improved by increasing its NaCl tolerance.

Knowledge, attitude and behavior on salt and health among primary school students in Jinan / 中国学校卫生

Objective@#To investigate the knowledge, attitude and practice(KAP) of salt and health among primary school students in Jinan, and to provide scientific evidence for targeted interventions.@*Methods@#1 293 primary school students of grade 4 in Jinan were randomly selected by cluster sampling method, and a self-designed questionnire on "salt and health" was applied among them.@*Results@#Among grade 4 students in Jinan, 35.73% were aware the diagnostic criteria of hypertension in adults, 67.67% were aware that eating less salt help lowering blood pressure. The proportion of students who knew the recommended daily intake of salt for adults, children aged 1-6 years and for children aged 7-13 years was 55.68%, 53.21% and 50.62%, respectively. The proportion of students who favor lower intake of salt(70.84%), actively choose low-salt food(65.89%), received publicity or education on low-salt diet(72.85%), pay attention to the salt/sodium content when purchasing packaged food(73.01%), believe that packaged food should be labelled with salt/sodium content(84.45%), and heard of low-sodium salt (alternative salt)(57.77%).The boys and girls were found no statistical significance in the differences of their attitude toward controlling salt, skills and behaviors(P<0.05),with 68.05% of boys and 73.91% of girls agreed, 13.76% of boys and 9.24% of girls reported no attention paid. There was no significant difference in other salt control attitudes, skills and behaviors between boys and girls(P>0.05).@*Conclusion@#A series of interventions taken by Shandong Ministry of Health Action on Salt Reduction and Hypertension (SMASH) within schools show positive effects. However, gaps in salt taste and salt intake among primary school students still exist, suggesting health education should be further strengthened in respect of daily salt intake recommendation, salt intake attitude and behaviors, especially for boys.

The prevalence and risk factors of metabolic syndrome in Chinese population based on the multi center cross-sectional survey / 中华疾病控制杂志

Objective To understand the prevalence and risk factors of metabolic syndrome (MS) among different ethnic groups. Methods A multicenter cross-sectional survey was conducted. Subjects were selected by multistage stratified random sampling. Physical examination and laboratory testing were performed to collect MS related indicators, and the prevalence was standardized by the 6th general survey data. Further multivariate and logarithmic linear model methods were applied to analyze the risk factors and interaction. Results The overall prevalence of MS was 19.58%. The highest prevalence of MS was in Korean, followed by Han, while the lowest was in Kazakh. The rates of MS, overweight and obesity were higher in men than those in women, and increased along with age. Multivariate analysis result showed that the odds ratio (OR) of female to male was 0.556, and aging increased the risk of MS. The OR of central obesity was 2.765, and would reach to 4.259 when the waist-to-body ratio was over 0.52. The logarithmic linear model showed that the overweight/obesity, hyperglycemia, hypertension and dyslipidemia had independent effects on the risk of MS. Also, there were interactions in the four indicators. Conclusions The incidence of MS is high and the positive interaction between the overweight/obesity, hyperglycemia, hypertension and dyslipidemia is observed, making MS a common crisis to clinical and public health. In order to prevent and control MS, and to reduce the risk of cardiovascular and cerebrovascular diseases and diabetes, early screening of MS should be strengthened and lifestyle intervention should be carried out.

Study on the Correlation between Barium Radiography and Pulmonary Infection rate in the Evaluation of Swallowing Function

OBJECTIVES: To compare the results respectively obtained from the utilization of 60% barium sulfate suspension and Iohexol as contrast agents for videofluoroscopic swallowing studies and the relationship between the clinical application of the two kinds of contrast agents and the incidence of pneumonia. METHODS: Sixty cases of stroke patients with dysphagia were selected in rehabilitation department of our hospital, and the gender, age, position of the disease, and stroke nature between groups had no significant difference. Among which, 30 patients who were administered 350 mgI/ml Iohexol, and the other 30 patients with 60% barium sulfate suspension as contrast agent. We performed videofluoroscopic swallowing studies with barium 60% versus Iohexol within 1 week after admission and 2 weeks after admission. RESULTS: After 2 weeks in hospital, the aspiration pneumonia incidence of two groups was statistically significant (p<0.05), the pneumonia incidence of Iohexol group was lower than barium sulfate group which might have a impossble relevance with barium aspiration. CONCLUSIONS: During the videofluoroscopic swallowing study of dysphagia after stroke, barium sulfate can enhance the pneumonia incidence, and Iohexol can be widely applied in videofluoroscopic swallowing study.

Metformin hydrochloride antagonizes glucotoxicity in osteoblasts by restraining the expression of PPARγ / 中国药学杂志

OBJECTIVE: To investigate the protective effects of metformin hydrochloride (MF) on osteoblasts which were exposed to constant high glucose condition, and the relationship between MF and the expression of peroxisome proliferator-activated receptor (PPAR)γ. METHODS: Mouse cranium osteoblasts were cultured in vitro and divided into 5.5 mmol · L-1 normal glucose +0 μmol · L-1 MF group, 25 mmol · L-1 high glucose with different concentration of MF (0, 25, 50, 100 μmol · L-1 MF, respectively) and intervened for 1,2 and 3 w, then the mRNA expression levels of PPARγ and bone morphogenetic protein (BMP)-2 were detected by RT-PCR, and the protein expression levels of PPARγ was examined by Western blot. RESULTS (1)The mRNA expression levels of PPARγ was marked higher in high glucose group compared to the control group with the same intervene time (all P < 0.05); when the intervention time prolonged to 3w, the protein expression of PPARγ increased significantly (P < 0.05); the mRNA expression levels of BMP-2 in the high glucose group was lower than in the control group (all P < 0.05). (2)At the same intervening time, the expression of PPARγ mRNA decreased while BMP-2 mRNA increased (all P < 0.05) with the increasing concentration of MF (from 0 μmol · L-1 to 100 μmol · L-1); when the intervention time prolonged from 1w to 3w, the mRNA expression levels of PPARγ increased in all the high glucose groups while the mRNA expression levels of BMP-2 decreased (all P < 0.05). (3)When interviewed for 3w, the protein expression of PPARγ decreased gradually with the increasing concentration of MF in high glucose condition (P < 0.05). CONCLUSION: Pathological concentration of glucose could lead to poorly differentiated of osteoblasts, which might association with the enhanced mRNA and protein expression levels of PPARγ; MF can protect osteoblasts by restraining the expression of PPARγ.

Relationship between body fat and beta-cell function in obesity women of PI-deficiency with phlegm-dampness type and QI-stagnancy with phlegm-blocking type / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To analyze the relationship between body fat and beta-cell function in obesity women of Pi-deficiency with phlegm-dampness type (PDPD) and qi-stagnancy with phlegm-blocking type (QSPB).</p><p><b>METHODS</b>Sixty women, who had normal blood glucose level and without family history of diabetes, were enrolled. They were classified into non-obesity group and obesity group depending on their body mass index (BMI), and subjects of obesity group were differentiated into the PDPD type and QSPB type according to Chinese medicine syndrome differentiation. The body fat was detected using double energy X-ray absorptiometry, and the beta-cell function was assessed by measuring the acute insulin response (AIR), the under insulin curve area (AUCins), the under glucose curve area (AUCglu), and their ratio (AUCins/AUCglu), through intravenous glucose tolerance test (IVGTT).</p><p><b>RESULTS</b>BMI, body fat and waist circumference (Wf) were higher in obesity subjects than those in non-obesity subjects, but showed no significant difference between the two obesity types. Comparisons between obesity women of different types showed that the fat content of trunk and total body, the ratio of trunk fat/total mass, AIR, AUCins, and AUCins/AUCglu were all higher in QSPB than those in PDPD. AIR, AUCins, AUCins/AUCglu showed good correlation with BMI, Wf, trunk fat and total body fat contents. Multiple linear regression analysis demonstrated the increasing of trunk fat content was an influencing factor of AIR.</p><p><b>CONCLUSION</b>Obesity women of QSPB type possess higher body fat (especially the trunk fat) content and insulin resistance with high acute insulin response, so clinical intervention should dominantly pay attention to subjects with QSPB type of obesity.</p>

Influences of Bis(maltolato) oxovanadium in kidney morphology in spontaneously hypertensive rats / 吉林大学学报(医学版)

Objective To study the effects of Bis(maltolato) oxovanadium(BMOV) on renal structure of spontaneously hypertensive rats(SHRs) and its mechanism.Methods Thirty-six SHRs were divided into three groups at random: blank group,Nifedipine group and BMOV group.The light microscope and electron microscope were used to examine the pathologic changes of the kidney in rats.Results The rat glomerular mesangial expansion was found and the lumen of glomeruler capillary nearly closed in bland group,while in BMOV group the pathological changes mentioned above were unobvious.Conclusion BMOV may have protective effects on kidney in SHRs.